negative binomial regression model with random effects stata 8.1 Search Results


99
ATCC vero cells
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
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Thermo Fisher tween 20
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Tween 20, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Millipore es cell characterization kit
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Es Cell Characterization Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
New England Biolabs tween 20
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Tween 20, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Cell Signaling Technology Inc anti vegfr2
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Anti Vegfr2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Santa Cruz Biotechnology tween 20
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Tween 20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Cole-Parmer ultrasonic bath cole parmer 8850
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Ultrasonic Bath Cole Parmer 8850, supplied by Cole-Parmer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Millipore tbst
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Tbst, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
New England Biolabs pcr rflp
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Pcr Rflp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Millipore tween 20
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
New England Biolabs saline tween 20
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Saline Tween 20, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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98
Bio-Rad pbst
Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with <t>Vero</t> <t>cells</t> for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Pbst, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with Vero cells for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.

Journal: Frontiers in Microbiology

Article Title: A Therapeutic Antiviral Antibody Inhibits the Anterograde Directed Neuron-to-Cell Spread of Herpes Simplex Virus and Protects against Ocular Disease

doi: 10.3389/fmicb.2017.02115

Figure Lengend Snippet: Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with Vero cells for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.

Article Snippet: Vero cells (American Type Culture Collection, ATCC, CCL81, Rockville, MD, United States) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Life Technologies Gibco, Darmstadt, Germany) containing 10% (v/v) FCS (Life Technologies Gibco), 100 U/ml penicillin, and 0.1 mg/ml streptomycin (penicillin–streptomycin).

Techniques: Infection, Eye Drops, Injection, Isolation, Cell Culture