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Image Search Results
Journal: Frontiers in Microbiology
Article Title: A Therapeutic Antiviral Antibody Inhibits the Anterograde Directed Neuron-to-Cell Spread of Herpes Simplex Virus and Protects against Ocular Disease
doi: 10.3389/fmicb.2017.02115
Figure Lengend Snippet: Reduced frequency of HSV reactivations from the trigeminal ganglia of antibody-treated mice. Mice ( n = 10) were infected with HSV-1 KOS. The antibodies mAb 2c or mAb hu2c were applied at 24 h prior to infection (pre-exposure prophylaxis, P), at 24, 40, and 56 h after infection (PEP) or at day 6 post-infection (therapy, T, only mAb 2c). Topical treatment with mAb 2c was started at 24 h post-infection and performed five times per day by applying antibody containing eye drops until day 12. ACV standard therapy at 10 mg/kg body weight was performed three times a day by intraperitoneal injection. The percentages of reactivating ipsilateral or contralateral trigeminal ganglia from HSV-1 KOS-infected mice are shown. Trigeminal ganglia were isolated on day 12 after infection and co-cultured with Vero cells for 4 weeks. The occurrence of cytopathic effects was equated as reactivation. The differences in the total numbers of reactivating ganglia between the antibody treatment groups were compared with PBS and statistical significances were determined with the Fisher’s exact test. Comparisons were considered significant at ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
Article Snippet:
Techniques: Infection, Eye Drops, Injection, Isolation, Cell Culture